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目 录 1.引言 .....................................................................................................................................1 http://www.paper51.com 2.实验部分 ...........................................................................................................................2 paper51.com 2.1实验原理 ............................................................................................................2 copyright paper51.com 2.2仪器及试剂 .......................................................................................................3 http://www.paper51.com 2.2.1实验仪器 .......................................................................................................3 copyright paper51.com 2.2.1实验药品及试剂..........................................................................................3 paper51.com
2.3 实验步骤 .............................................................................................................3 http://www.paper51.com
2.3.1药材前处理 .............................................................................................3 内容来自www.paper51.com 2.3.2药材浸泡 .......................................................................................................3 http://www.paper51.com 2.3.3提取和过滤.....................................................................................................3 http://www.paper51.com
2.3.4粗样品的环肽粗步检测.................................................................................3 copyright paper51.com
2.3.4.1点样和展开..........................................................................................3 内容来自www.paper51.com
2.3.4.2环肽的初步检测..................................................................................4 内容来自论文无忧网 www.paper51.com 2.3.4.3初步检测结果及评估.……………………………….………………4 paper51.com 2.3.5样品分离和提纯.............................................................................................4 内容来自www.paper51.com 2.3.6环肽部位的确定.............................................................................................5 paper51.com 2.3.7 实验条件选择................................................................................................5 paper51.com 2.3.7.1展开剂的比例选择..............................................................................5 http://www.paper51.com 1.第一向展开剂选择...............................................................................5 paper51.com 2.第二向展开剂选择...............................................................................5 内容来自论文无忧网 www.paper51.com 2.3.7.2水解时间的择......................................................................................5 paper51.com 2.3.7.3水解时酸的种类择..............................................................................5 paper51.com 2.3.8样品环肽检测.................................................................................................6 paper51.com
2.3.8.1第一向展开 ........................................................................................6 内容来自www.paper51.com 2.3.8.2环肽检测 ............................................................................................6 内容来自www.paper51.com 2.3.8.3第二向展开和检测 ............................................................................6 http://www.paper51.com
3结果与讨论...........................................................................................................................6 内容来自www.paper51.com
3.1瞿麦中环肽检测结果及讨论....................................................................................7 内容来自www.paper51.com 3.2 太子参中环肽检测结果及讨论...............................................................................8 http://www.paper51.com
3.3 马鞭草中环肽检测结果及讨论...............................................................................9 paper51.com 3.4 苍术中环肽检测结果及讨论 ...............................................................................9 内容来自论文无忧网 www.paper51.com 4.实验过程中存在的其它问题...............................................................................................9 内容来自论文无忧网 www.paper51.com
结语..........................................................................................................................................9 http://www.paper51.com
致谢........................................................................................................................................10 copyright paper51.com 参考文献 ............................................................................................................................11 paper51.com
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